Expertise

The SFMB lab has a long history of experience in the field of membranes and lipids since the early seventies.

The main fields of expertise are currently:

  • expression, purification and crystallization of membrane proteins.
  • reconstitution of membranes from lipids and proteins
  • role of lipids in modulation of membrane protein activity
  • FTIR spectroscopy and imaging of proteins, cells and tissues
  • electrophysiology, membrane interacting peptides and ion channels reconstitution in planar lipid bilayers.
  • computer modellisation of lipid-protein interaction

Facilities :

  • 2 cell culture facilities for eucaryotic cells
  • one L2 room for micro-organism culture with medium-sized fermentors
  • several FTIR spetrophotometers equipped with ATR sample holder
  • two FTIR microscopes
  • 1 crystallisation robot
  • 2 HPLC for proteins
  • 1 "research-grade" fluorimeter (PTI)
  • 2 luminometers
  • 1 Malvern particle sizer (PCS-DLS)
  • 1 isothermal titration calorimeter
  • 1D and 2D electrophoresis
  • 1 multiwell plate-reader (fluoresence, luminescence and absorbance)
  • 1 ESI MS/MS mass spectrometer
  • 1 nanodrop and 1 conventionnal spectrophotometer
  • 1 computer cluster for molecular dynamic simulations
  • 3 electrophysiology setup